Oligonucleotides for cDNA libraries¶
Name |
Sequence (5’ → 3’) |
Comment |
|---|---|---|
P5 |
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT |
PCR-primer |
BC1 |
CAAGCAGAAGACGGCATACGAGAT_CGTGAT_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC2 |
CAAGCAGAAGACGGCATACGAGAT_ACATCG_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC3 |
CAAGCAGAAGACGGCATACGAGAT_GCCTAA_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC4 |
CAAGCAGAAGACGGCATACGAGAT_TGGTCA_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC5 |
CAAGCAGAAGACGGCATACGAGAT_CACTGT_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC7 |
CAAGCAGAAGACGGCATACGAGAT_CAGATC_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
BC9 |
CAAGCAGAAGACGGCATACGAGAT_GATCAG_GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT |
|
App-PE |
AppNAGATCGGAAGAGCACACGTCTG-ddC |
3’-linker |
L5Aa |
invddT_ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrUrArArGrC-OH |
5’-linker |
L5Ac |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrGrCrGrCrArGrC-OH |
5’-linker |
L5Ad |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrCrGrCrUrUrArGrC-OH |
5’-linker |
L5Bb |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrGrUrGrArGrCrN-OH |
5’-linker |
L5Bc |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrCrArCrUrArGrCrN-OH |
5’-linker |
L5Da |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrCrGrUrGrArUrN-OH |
5’-linker |
L5Db |
invddT-ACACrGrArCrGrCrUrCrUrUrCrCrGrArUrCrUrNrNrNrGrCrArCrUrArN-OH |
5’-linker |
PE-rev |
CAGACGTGTGCTCTTCCGATCT |
RT-primer |