snRcne03050-SNORD30

../../_images/snRcne03050_h99_igb.png

  • snRcne03050-SNORD30_intronic-boxCDsnoRNA-in-CNE03050 (CNAG_02236)

  • Within intron of CNE03050 (CNAG_02236) for Serine/threonine-protein phosphatase

  • Predicted target of the D’ guide is in 25S rRNA: gttCtctttt and appears unique for Tremellomycetes

../../_images/25Sdom3_cne03050-target.svg

  • The cytosine predicted to be modified is not present in yeast; substitutions cf. yeast 25S rRNA are in blue (change of purine or pyrimidine) or red (change from purine to pyrimidine or vice versa).

  • Poorly conserved D guide appears orthologous to, with respect to their D guides, plant snoZ103, or human SNORD30. This guide has complimentarity to many U-rich loci.

  • Complimentarity of D-guide tttcCgt with 18S (1774-1780) and two 25S loci (167-173, 1591-1597), seems too short to support methylation; targeted nucleotides are not described as modified in plant or human (human SNORD30 seems only functional via the D’ guide which differs in its 25S target from that of Tremellomycetes).

../../_images/snRcne03050-align.png

>AE017345.1:865876-866011 Cryptococcus neoformans var. neoformans JEC21 chromosome 5 sequence
GCGTGTGAAGAAAAATACAAAAGAGGACCCTGAGGCTTTTGGGCCTGTGGAGATGGCTTACTTGGTGGGT
CCGAGGTACGCTCATATGAGAAACCCGCCACCCGGTGAGCACGAGAGAACGGAAATCTGATGCTCA







  • Putative targets in C. deneoformans, aligned to homologous sequences found in Tremellomycetes.

  • No significant conservation present that would support a role for modification by the snoRNA.

  • Only chromosomes 1 and 2 have been checked, and only for candidate targets with potential to base-pair to the full guide:

Tremellomycetes targeted segments
   

../../_images/CNA00570-align.png

CNA00570 (IFVFF motif), CNAG_00066

 

 

../../_images/CNA04450-tRNA-terminator-align.png

Terminator for tRNAs CNA04450, CNA04530

   

../../_images/CNA07430-5utr-align.png

5’ UTR CNA07430, CNAG_00765

   

../../_images/vnEBG00005237514-tRNA-terminator-not-conserved.png

Terminator tRNA vnEBG00005237514

   

../../_images/CNB00360-align.png

3’UTR CNB00360, CNAG_06765

   

../../_images/CNB01100-align.png

3’SS intron CNB01100, CNAG_03607

   

../../_images/CNB02730-align.png

intron CNB02730, CNAG_07481

   

../../_images/CNB03400-align.png

intron 5’SS CNB03400, CNAG_03850 (SPT7)

   

../../_images/CNB04310-align.png

intron 5’SS CNB04310, CNAG_03944

  • Image source snRcn03050:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
Annot/1-39                        ------RTGATGA---------UUUUCUCUUG-CTGA---------------------RTGATGA----------------------------------------------------------------------------------------UGCCUUU-CTGA------
#=GS snRcne03050-SNORD30/1-136 DE intronic-boxCDsnoRNA-in-CNE03050 AE017345.1:865876-866011 Cryptococcus neoformans var. neoformans JEC21 chromosome 5 sequence
snRcne03050-SNORD30/1-136         --GCGTGTGAAGAAAA--ATACAAAAGAGGACCCTGAGGCT------TTTG-----GGCCTGTGGAGATG--GCTTACTT-GGTGG----GTC---CG-AGGTACGCTC----ATAT----GAGAAACCCGCCACC-CGGTGAGCACGAGAGAACGGAAATCTGATGCTCA
#=GS in-CNAG_02236/1-136 DE CP003825.1:833424-833559 in CNAG_02236 Serine/threonine-protein phosphatase Cryptococcus neoformans var. grubii H99 chromosome 6, complete sequence
in-CNAG_02236/1-136               --GCGTGTGAAGAAAA--ATACAAAAGAGGACTCTGAGGCT------TTTG-----AGCCTGTGGAGATG--GCTTACTT-GGTGG----GTC---CG-AGGTACGCTC----ATAC----GAGAAACCCGCCACC-AGGTGAGCAAGAGAGAACGGAAATCTGATGCTCA
#=GS WM276/1-135 DE CP000290.1:768442-768576 Cryptococcus gattii WM276 chromosome E, complete sequence
WM276/1-135                       --GCGTGTGAAGAAAA--ATACAAAAGAGGACTCTGAGGCT------TTA------GGCCCATGGAGATG--GCTTACTT-GGTGG----GTC---CG-AGGTACGCTC----ATAT----GAGAAACCCGCCACT-AGGTGAGCACGAGAGAACGGAAATCTGATGCTCA
#=GS R265/1-135 DE CP025764.1:c566066-565932 Cryptococcus gattii VGII R265 chromosome 6, complete sequence
R265/1-135                        --GCGTGTGAAGAAAA--ATACAAAAGAGGACTCTGAGGCT------TTA------GGCCCATGGAGATG--GCTTACTT-GGTGG----GTC---CG-AGGTACGCTC----ATAT----GAGAAACCCGCCACT-AGGTGAGCAAGAGAGAACGGAAATCTGATGCTCA
#=GS KwoSha/1-135 DE NQVO01000022.1:16297-16431 Kwoniella shandongensis strain CBS 12478 scaffold00022, whole genome shotgun sequence
KwoSha/1-135                      --GCGCGTGATGACAA-ACCGCAAAAGAGGACTCTGAGGC-------ATT-------GCCTATGGAGAATA-GTCTGTCTCTGTGG----GAC---CG-AGGTATACTC-----TCC----GAGGAACCCGCCGCGGAGGTGGATAAGAGAGAACGGAAATCTGATGCTC-
#=GS TreMes/1-133 DE SDIL01000011.1:55446-55579 Tremella mesenterica strain ATCC 28783 supercont1.11, whole genome shotgun sequence
TreMes/1-133                      --GCATATGATGAGAA--TTTCAAAAGAGGACTCTGAGGCT------TTCG-----AGCCAATGGATAGCC-CTTCACT--TCTCG----AA----TG-TGGTACGCTC-----AGA----GAGAAACCCACGAGA--GGTGAAGAAGAGAGAACGGAAATCTGATGCTTG
#=GS CryFlo/1-141 DE RRZH01000004.1:995080-995220 Cryptococcus floricola strain DSM 27421 chromosome 4, whole genome shotgun sequence
CryFlo/1-141                      --GCGTGTGATGAGA---AAACAAAAGAGGACTCTGAGGCC------GCTG-------CCTATGGAGAAG--GCTCTGCTGGTCGG-----C----CGTCGGTACACTGGCAAGTCTTTGCAAGGAACCCGCTGTCCAGGAGAGT-ATAGAGAACGGAAATCTGATGCTCA
#=GS CryWin/1-141 DE AWGH01000041.1:33202-33342 Cryptococcus wingfieldii CBS 7118 supercont1.41, whole genome shotgun sequence
CryWin/1-141                      --GCGTGTGATGAGA---AAACAAAAGAGGACTCTGAGGCC------GCTG-------CCTATGGAGAAG--GCTCTGCTGGTCAG-----C----CGTCGGTACACTGGCAAGTCTTTGCAAGGAACCCGCTGTCCAGGAGAGT-ATAGAGAACGGAAATCTGATGCTCA
#=GS CryAmy/1-141 DE MEKH01000006.1:c696754-696614 Cryptococcus amylolentus CBS 6273 supercont2.6, whole genome shotgun sequence
CryAmy/1-141                      --GCGTGTGATGAGA---AAACAAAAGAGGACTCTGAGGCCC------CT-------GCCAATGGAGAAG--GCTCTGCTGGTCAG-----C----CGTAGGTACACTGGCAAGTCTTTGCAAGGAACCCGCTGTTCAGGAGAGT-ATAGAGAACGGAAATCTGATGCTCA
#=GS TreTag/1-138 DE CAJHEQ010000293.1:c4285-4148 MAG: Tremellales sp. Tagirdzhanova-0007 genome assembly, contig: TREM_293, whole genome shotgun sequence
TreTag/1-138                      --GCGCGTGATGAAAAA-TTTCAAAAGAGGCTTCCGATGCC-----ACCG------GGCACTGGGGAATGGTCTGATTCTCGCGG-----GG----CAGTGGTATACTC----GACA----GAGGAACCCG--CCGCGGGAACAGACGAGAGAACGGAAATCTGATGCTAG
#=GS TreYok/1-141 DE BRDC01000032.1:c261738-261596 Tremella yokohamensis NBRC 100148 DNA, KCNB35TY.32, whole genome shotgun sequence
TreYok/1-141                      --GCAAGTGATGAGAGACTTTCAAAAGAGGACTCTGAGGCCTT-----TC----GGGGTCAATGGACAGCC-CTCCGCGTT-------CAGTC-GCAG-TGGTACACTG-----TGT----CAGGAACCCTGCCACGGCGCGGAGAAGAGAGAACGGAAATCTGATGCTCG
#=GS TreFuc/1-141 DE BRDD01000128.1:23272-23416 Tremella fuciformis NBRC 9317 DNA, KCNB80TF.128, whole genome shotgun sequence
TreFuc/1-141                      --GCAAGTGATGAGAAACTTTCAAAAGAGGACTCTGAGGCCT----TTCG-----GGGTCAATGGACAGCC-CTCCGCGCC-------CAGTC-GCAG-TGGTACACTG-----TGT----CAGGAACCCTGCGGCGGCGCGGAGAAGAGAGAACGGAAATCTGATGCTCG
#=GS KwoHev/1-129 DE ASQB01000002.1:c654959-654800 Kwoniella heveanensis BCC8398 cont1.2, whole genome shotgun sequence
KwoHev/1-129                      --GCGTGTGATGAAGAAC-TTCAAAAGAGGACTCTGAGGC-----TTTCTC------GCCAATGTAGTT---ACCCATCC--CGC-----GTC---TG-TGGTATACTT----CCTC----AAGGAACCCG--GTG--GGATGGGC--AGAGAACGGTTATCTGATGCTGG
#=GS KwoMan/1-144 DE ASQF01000015.1:c163924-163781 Kwoniella mangroviensis CBS 8886 cont1.15, whole genome shotgun sequence
KwoMan/1-144                      --GCGCGTGATGACAAAC-TTCAAAAGAGGACTCTGAGGC-----TCTTTC------GCCAATGGAGAACAAATCCGTTCCTATGT----GA----CAGTGGTATGCTC--CGTGAAGA--GAGAAACCCGGCGTAGGAACGGAT-AGAGAGAACGGAAATCTGATGTGAA
#=GS CrySki/1-148 DE BCHT01000005.1:426613-426760 Cryptococcus skinneri DNA, scaffold: scaffold_4, strain: JCM 9039, whole genome shotgun sequence
CrySki/1-148                      --GCGTGTGATGATGACCA-TCAAAAGAGGACTCTGAGGTCTTGA-TAAAT-TCGAGGTTGATGGAGAATG-GTCCGTACTCTTGAT---GCC----G-TGGTATACTC-----TCC----GAGGAACCCTCCAAGAGTGCGGATACGAGAGAACGGAAATCTGATGCTTG
#=GS NaeEnc/1-135 DE MCFC01000001.1:c594919-594782 Naematelia encephala strain 68-887.2 BCR39scaffold_1, whole genome shotgun sequence
NaeEnc/1-135                      --GCGCGTGATGAAATCC--TCAAAAGAGGCTTAAGAGAC------TGTC-------GTCGATGAGTTTG--GCCCT-GTTCGTGGGG--A-----CAGCGGTACACTC-----TGC----GAGGAACCCGCCCCAAGAAAGGGCTCGAGAGAACGGAAATCTGATGCCAT
#=GS NaeAur/1-136 DE JAKFAO010000006.1:c1133444-1133309 Naematelia aurantialba strain NX-20 Contig6, whole genome shotgun sequence
NaeAur/1-136                      --GCGTGTGATGAGACCC--TCAAAAGAGGCTTAAGAGGCG------GT-------CGCCAATGAGGTTG--GCCCTT-CACTTGGGG--ATA----G-AGGTACACTCG----GG----CGAGGAACCCGTCCCGAGCAAGGGCTCGAGAGAACGGAAATCTGATGCCAT
#=GS HomSap-SNORD30/1-70 DE Homo_sapiens SNORD30 http://snoopy.med.miyazaki-u.ac.jp/snorna_db.cgi?mode=sno_info&id=Homo_sapiens300268
HomSap-SNORD30/1-70               --ATACATGATGACTTACATGGACTCTCATTCAGCTAATG-----ACTTG--------CTGCTGAA-------------------------------------------------------------------------------------ACATGGAAATCTGATTTTT-
#=GS MyoLuc-SNORD30/1-70 DE Myotis_lucifugus SNORD30 28S rRNA 28S:A3136 Little brown bat http://snoopy.med.miyazaki-u.ac.jp/snorna_db.cgi?mode=sno_info&id=Hordeum_vulgare300077
MyoLuc-SNORD30/1-70               --ATATATGATGACTGACATGGAATCTCGTTCGGCTGATG-----ACTTG--------CTGTTGAG-------------------------------------------------------------------------------------ACTCGGAAATCTGATTTTC-
MyoLuc-guide/1-16                 ---------------------GAATCTCGTTCG-CTGA-------------------------------------------------------------------------------------------------------------------------------------
#=GS snoZ103/1-95 DE Hordeum_vulgare snoZ103 http://snoopy.med.miyazaki-u.ac.jp/snorna_db.cgi?mode=sno_info&id=Hordeum_vulgare300077
snoZ103/1-95                      ATGGACCCGAGGA-TACTGTCCCCAAATTATCGACTATGAGTAT--ACA----ATGCGCAAATGT-------AATCC------------------------------------CATCA----------------------GGATT---AAAAGCCGGAAATCTGATCCAT-
#=GC SS_cons                      --(((........--..........-------((..(((((-....----------)))))..)).....--(((((((.-(((((----...---((-.(((...(((----....----)))..))))))))))-.))))))).............-------)))...
//

  • Image source CNA00570, CNAG_00066:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
#=GS Found-target/1-13 DE 1:159894-159903:-1 CNA00570
Found-target/1-13         --------------------------------------------------------------ATTTTTGTTTTCT----------------------------------------------------
snRcne03050/1-17          ---------------------------------------------------------AGTC-TAAAGGCAAGAGA----------------------------------------------------
#=GS CNA00570/1-127 DE XM_024656150.1:2209-2335 Cryptococcus neoformans var. neoformans JEC21 DNA-binding protein cre-1, putative (CNA00570), mRNA
CNA00570/1-127            TATCCATGCCCGGAACAGCTGCCGATTGGATCACCTATGCCATCCTCATCATCCAAGCAATCATTTTTGTTTTCTTCCTCTTGATGCTCCTCACTAAAATCATCGAAGGTTCTATTCGTCTCTTCGG
#=GS CNAG_00066/1-127 DE CP003820.1:c181263-181137 Cryptococcus neoformans var. grubii H99 chromosome 1, complete sequence
CNAG_00066/1-127          TATCGATGCCGGGAACAGCTGCCGATTGGATCGCCTATGCCATCCTCATCATCCAAGCAATCATATTTGTCTTCTTTCTCTTGATGCTTCTCACCAAAATCATCGAAGGTTCTATTCGTCTCTTCGG
#=GS R265/1-127 DE CP025762.1:c150369-150243 Cryptococcus gattii VGII R265 chromosome 4
R265/1-127                TATCAATGGCCTCCACAGCTGCCGATTGGATCACCTACGCCATCCTCATTGTCCAAGCAATCATCTTTGTCTTCTTCCTCCTCATGCTCCTCACCAAACTCATCGAAGGTTTTATCCGTCTCTTTGG
#=GS WM276/1-127 DE XM_003191840.1:2006-2132 Cryptococcus gattii WM276 Membrane protein, putative (CGB_B0600C), partial mRNA
WM276/1-127               TATCAATGGCCTCCACAGCTGCCGACTGGATCACCTACGCCATCCTCATTGTCCAAGCAATCATCTTTGTCTTCTTCCTCCTCATGCTCCTCACCAAACTCATCGAAGGTTCTATCCGTCTCTTTGG
#=GS CryWin/1-104 DE CP034265.1:1354403-1354506 Cryptococcus wingfieldii strain CBS7118 chromosome 5, complete sequence
CryWin/1-104              -----------------------GACTGGATAGCCTACGCCGTCCTCATCCTCCAAGCAATCATATTCGTATTTTTCCTCTTTATGCTCTTTACCAAGATCATCGAGGGCTCCATCCGGCTGTTTGG
#=GS CryAmy/1-104 DE XM_019137732.1:2188-2291 Cryptococcus amylolentus CBS 6039 hypothetical protein (L202_03800), mRNA
CryAmy/1-104              -----------------------GACTGGATAGCCTACGCCGTCCTCATCCTCCAAGCAATCATATTCGTGTTTTTCCTCTTTATGCTCTTTACCAAGATCATCGAGGGCTCCATCCGGCTGTTTGG
#=GS KwoSha/1-127 DE NQVO01000063.1:11835-11961 Kwoniella shandongensis strain CBS 12478 scaffold00063, whole genome shotgun sequence
KwoSha/1-127              TATCCATGACCTGGTCCGCAGTGGCTTGGATCACTTACGCCATCCTCATCATCCAAGCGATTATCTTCCTCTTCTTCATTCTCATGCTCCTTACCAAGATCATCGAAGGTCTCATTCGACTCTTTGG
#=GS CryFlo/1-104 DE RRZH01000005.1:c263822-263719 Cryptococcus floricola strain DSM 27421 chromosome 5, whole genome shotgun sequence
CryFlo/1-104              -----------------------GACTGGATAGCCTACGCCGTCCTCATCCTCCAAGCAATCATATTCGTATTTTTCCTCTTTATGCTCTTTACCAAGATCATCGAGGGCTCCATCCGGCTGTTTGG
#=GS KwoHev/1-104 DE ASQC01000038.1:11647-11750 Kwoniella heveanensis CBS 569 cont2.38, whole genome shotgun sequence
KwoHev/1-104              -----------------------GATTGGATCGCATACGCTGTGCTCATCCTCCAAGCAATCACTTTCCTCTTCTTCCTCTTCATGCTCCTTACCAAGATAATCGAAGGCGCAATCCGAATCTTCGG
#=GS NaeEnc/1-105 DE MCFC01000009.1:c176245-176141 Naematelia encephala strain 68-887.2 BCR39scaffold_9, whole genome shotgun sequence
NaeEnc/1-105              ----------------------CGACTGGCTTGCGTACGCCATTCTTCTTCTTCAAGCCATCACCTTTGTCTTCTTCCTTGTGATACTTCTCACGAAAATCACCGAGGGTCTAATTCGTCTCTTTGG
#=GS PapTer/1-104 DE JAHXHD010000012.1:20614-20717 Papiliotrema terrestris strain LS28 scaffold-11, whole genome shotgun sequence
PapTer/1-104              -----------------------GACTGGATCACCTACGCCATTCTCATCCTTCACGCGATCGTCTTCGTCTTCTTCGTCCTCATGCTCATGACGAAGCTCGTCGAGGGTCTCATCCGGCTCGTCGG
//

  • Image source CNA04450 ; CNA04530:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
#=GS Found-target/1-13 DE 1:1199404-1199417:-1  CNA04450-tRNA-terminator
Found-target/1-13                        -------------------------------------------------------------A-TTTTTGTTCTTT----
snRcne03050/1-17                         --------------------------------------------------------AGTC-T-AAAGGCAAGAGA----
#=GS CNA04450/1-74 DE AE017341.1:c1199477-1199404 Cryptococcus neoformans var. neoformans JEC21 chromosome 1, complete sequence
CNA04450/1-74                            TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-A-TTTTTGTTCTTTT---
#=GS CNA04530/1-74 DE  AE017341.1:1208566-1208639 Cryptococcus neoformans var. neoformans JEC21 chromosome 1, complete sequence
CNA04530/1-74                            TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-A-TTTTTGTTCTTTT---
#=GS AE017341.1:c1197656-1197584/1-73 DE Cryptococcus neoformans var. neoformans JEC21 chromosome 1, complete sequence
AE017341.1:c1197656-1197584/1-73         --CAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAATAT-TTTTTGTTCTTTT---
#=GS R265/1-72 DE CP025761.1:1045533-1045604 Cryptococcus gattii VGII R265 chromosome 3, complete sequence
R265/1-72                                --CAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-A-TTTTTGTTTTTTT---
#=GS H99/1-74 DE CP003820.1:c1207325-1207252 Cryptococcus neoformans var. grubii H99 chromosome 1, complete sequence
H99/1-74                                 TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAGT-A-TTTTTGCTCTTTT---
#=GS H99/1-76 DE CP003820.1:c1207865-1207790 Cryptococcus neoformans var. grubii H99 chromosome 1, complete sequence
H99/1-76                                 TTAAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAATATTTTTTTGTTCTTTT---
#=GS H99/1-76 DE CP003820.1:1217037-1217112 Cryptococcus neoformans var. grubii H99 chromosome 1, complete sequence
H99/1-76                                 TTAAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAATTCTTTTTTGTTCTTTT---
#=GS WM276/1-67 DE CP000286.1:c936199-936125 Cryptococcus gattii WM276 chromosome A, complete sequence
WM276/1-67                               TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAATA--TTTTTG----------
#=GS WM276/1-72 DE CP000286.1:947134-947205 Cryptococcus gattii WM276 chromosome A, complete sequence
WM276/1-72                               TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAAATCTTTTTGTTC-------
#=GS WM276/1-71 DE CP000286.1:c938017-937947 Cryptococcus gattii WM276 chromosome A, complete sequence
WM276/1-71                               --CAACACTAAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAATCTTTTTTGTTTT------
#=GS R265/1-74 DE CP025763.1:1104309-1104382 Cryptococcus gattii VGII R265 chromosome 5, complete sequence
R265/1-74                                TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAAATCTTTTTGCTTTT-----
#=GS R265/1-78 DE CP025761.1:1042848-1042925 Cryptococcus gattii VGII R265 chromosome 3, complete sequence
R265/1-78                                TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAA-TCTTTTTGGTTTTATCCC
#=GS AE017346.1:c232077-232000/1-78 DE Cryptococcus neoformans var. neoformans JEC21 chromosome 6 sequence
AE017346.1:c232077-232000/1-78           TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-TCTTTTTGTTTTGTTTTC
#=GS CP003826.1:1165914-1165987/1-74 DE Cryptococcus neoformans var. grubii H99 chromosome 7, complete sequence
CP003826.1:1165914-1165987/1-74          TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAGT-TCTTTTTGTTTTTT----
#=GS R265/1-72 DE CP025761.1:c1033757-1033686 Cryptococcus gattii VGII R265 chromosome 3, complete sequence
R265/1-72                                TTCAACACTGAAAGCCCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAAATCTTTTTGTTC-------
#=GS CP003828.1:c317256-317199/1-58 DE Cryptococcus neoformans var. grubii H99 chromosome 9, complete sequence
CP003828.1:c317256-317199/1-58           ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-A-TTTTTGTTTTTGA---
#=GS AE017349.1:c964343-964284/1-60 DE Cryptococcus neoformans var. neoformans JEC21 chromosome 9 sequence
AE017349.1:c964343-964284/1-60           ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAGT-A-TTTTTGTTTTTTGTT-
#=GS CP003822.1:c959240-959181/1-60 DE Cryptococcus neoformans var. grubii H99 chromosome 3, complete sequence
CP003822.1:c959240-959181/1-60           ----------AAGCACCGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT-TCTTTTTGTT--------
#=GS CP000293.1:958546-958604/1-59 DE Cryptococcus gattii WM276 chromosome H, complete sequence
CP000293.1:958546-958604/1-59            ----------------CGGCAGTCCTACGGGGCGTGGGTTCGAATCCCACTCTCGTCAAT--TTTTTTGTTTTGATT--
#=GS R265/1-57 DE CP025759.1:c443567-443511 Cryptococcus gattii VGII R265 chromosome 1, complete sequence
R265/1-57                                ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAG-TTTTTTTGTTTTT-----
#=GS WM276/1-58 DE CP000291.1:1100335-1100392 Cryptococcus gattii WM276 chromosome F, complete sequence
WM276/1-58                               ----------------CGGCAGTCCCCCGGGGCGTGGGTTCGAATCCCACTCTCGTCAAAATCTTTTTCCTTTT-----
#=GS CryWin/1-61 DE CP034272.1:345626-345686 Cryptococcus wingfieldii strain CBS7118 chromosome 12, complete sequence
CryWin/1-61                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATAC-TTTTTTGCATTCTTTT-
#=GS CryWin/1-58 DE CP034272.1:c262455-262398 Cryptococcus wingfieldii strain CBS7118 chromosome 12, complete sequence
CryWin/1-58                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATAC--TTTTTGCTTTTTG---
#=GS CryWin/1-59 DE CP034272.1:c286388-286330 Cryptococcus wingfieldii strain CBS7118 chromosome 12, complete sequence
CryWin/1-59                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATAC--TTTTTGCTTTTTAC--
#=GS CryWin/1-60 DE CP034272.1:c307915-307856 Cryptococcus wingfieldii strain CBS7118 chromosome 12, complete sequence
CryWin/1-60                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATAC--TTTTTGCTTTTTGCC-
#=GS CryWin/1-60 DE CP034272.1:c356137-356078 Cryptococcus wingfieldii strain CBS7118 chromosome 12, complete sequence
CryWin/1-60                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATAC--TTTTTGCTTTTTGCC-
#=GS CryFlo/1-57 DE RRZH01000013.1:377300-377356 Cryptococcus floricola strain DSM 27421 chromosome 12, whole genome shotgun sequence
CryFlo/1-57                              ----------------CGGCAGTCCTCCGGGGCGTGGGTTCGAATCCCACTCTCGTCATTC--TTTTTGCTTTTT----
//

  • Image source CNA07430, CNAG_00765:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
#=GS Found-target/1-13 DE 1:2037218-2037230:-1 5'UTR-exon
Found-target/1-13         ----------------------------------------------------------------GTTTCTGTTCTCT-----------------------------------------------------------
snRcne03050/1-17          -----------------------------------------------------------AGTC-TAAAGGCAAGAGA-----------------------------------------------------------
#=GS CNA07430/1-135 DE AE017341.1:c2037294-2037160 Cryptococcus neoformans var. neoformans JEC21 chromosome 1, complete sequence
CNA07430/1-135            GATTCGCATGTTTGATTTGTAGTTATTTTTTCCGGGAAGTGCGTACTCAGAACTGTCTTTCCACGTTTCTGTTCTCTCATCTCTGGCTTCATCTTCTCGCTCATCCTG-GTTCTCAGGAGTCTCTTCTTTTCTCAC
#=GS CNAG_00765/1-127 DE XM_012191556.1:75-201 Cryptococcus neoformans var. grubii H99 hypothetical protein (CNAG_00765), mRNA
CNAG_00765/1-127          GATTAGCATGTTTGATTTGTAGTTGTTTTTTCCGACAAGTACGTACT-------GTTTTCCCTCGTCTCTGTTCTCTGGTCTTTGGCTTCATCTTCTCGCTCATCCTGTGTTCTCAGAAGCCTCTTCTTTTCTC--
//

  • Image source tRNA vnEBG00005237514:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
#=GS Found-target/1-13 DE tRNA terminator
Found-target/1-13             -------------------------------------------------------------------------GTTTTTGTTTTTT----------
snRcne03050/1-17              --------------------------------------------------------------------AGTC-TAAAGGCAAGAGA----------
#=GS vnEBG00005237514/1-93 DE AE017342.1:c79232-79140 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
vnEBG00005237514/1-93         CGATTGCAATTTTATA---TTTGTAATCATCGGTAGGTCGTGGGTTCGAATCCCACTTCCCACTTTTTTCCTTGTTTTTGTTTTTTTAGTAGTCGA
#=GS CNAG_10144/1-80 DE CP003832.1:471071-471150 Cryptococcus neoformans var. grubii H99 chromosome 13, complete sequence
CNAG_10144/1-80               CGATTGCAATTTTACA----TCGCAGTTATCGGTAGGTCGTGGGTTCGAATCCCACTTCCCACTGTGTTTTTATTTCAATAAAT------------
#=GS R265/1-84 DE CP025762.1:1670939-1671022 Cryptococcus gattii VGII R265 chromosome 4
R265/1-84                     CGATTGCAATTTTA-----TTCGCAATCATCGGTAGGTCGTGGGTTCGAATCCCATTTCCCACTTTTATTATTTTTTTTTGGTTCTTTA-------
#=GS CNAG_10020/1-81 DE CP003821.1:c78956-78876 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_10020/1-81               CGATTGCAATTCTACAAAATTTGTAATTATCGGTAGGTCGTGGGTTCGAATCCCACTTCCCACTTTTTTCATCTTTTTTTT---------------
#=GS CNAG_10143/1-82 DE CP003832.1:470910-470991 Cryptococcus neoformans var. grubii H99 chromosome 13, complete sequence
CNAG_10143/1-82               CGATTGCAATTA-------TTTGTAATCATCGGTAGGTCGTGGGTTCGAATCCCACTTCCCACTTTCTTTTTATGTGTTTAGCCCAAAA-------
#=GS CNN01580/1-72 DE AE017356.1:461031-461102 Cryptococcus neoformans var. neoformans JEC21 chromosome 14 sequence
CNN01580/1-72                 CGATTGCAATTA-------TTTGTAATCATCGGTAGGTCGTGGGTTCGAATCCCACTTCCCACTTTCTTTTTCATGGGG-----------------
//

  • Image source CNB00360, CNAG_06765:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
#=GS Found-target/1-13 3'UTR
Found-target/1-13            --------------------------------------------------------------------------------------------GTTTTTGTTTTCT--------------------------------------------------------------------
snRcne03050/1-17             ---------------------------------------------------------------------------------------AGTC-TAAAGGCAAGAGA--------------------------------------------------------------------
#=GS CNB00360-3'UTR/1-189 DE AE017342.1:104660-104849 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
CNB00360-3'UTR/1-189         TACTCTTGTGTCTTTCTACTATTTGTCTTTTTTCTTTCTGCTTTACATTCATCAGTCACTGGTCTTTCCATATACCCATTCATCTTTCTTGCGTTTTTGTTTTCTTTTT-CA----TGGTAATGATCTCTCTT-GTCAGGTGT-TATCGGTCAATATTCAATATATAATGATT
#=GS CNAG_06765/1-189 DE LisH domain-containing protein CP003821.1:103159-103348 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_06765/1-189             TACTCTTGAGTTTTTCTA---TTTGTCTTTTTTCTCTCGGCTCTACAATCGTCAGTCAGGGG-CTTTCCATATACCCACCCATCTTTCGTGCTTTTGTCTTCTTTTT---CATTAATGGTAATGATCTCTCTTCGTCAGGTGTTTATTGGTCAATATTCAATATATAATGATT
#=GS R265/1-191 DE CP025762.1:c1646182-1645991 Cryptococcus gattii VGII R265 chromosome 4
R265/1-191                   ATCACTTTGTGTCTTCTGTCT---GTCTTTTCTCGCTCGGCTCTTCA----TCAGTCACTCGTCTTTCCATATACCCATCTATCCTTCGTGTGCTTTTGTGTCTCTTTTTCATCAATGGTAATGATCTCTCTTTGTCAGGTGTTT-TCGGTCAATATTCAATATATAATGATT
#=GS WM276/2-192 DE CP000287.1:c2073856-2073665 Cryptococcus gattii WM276 chromosome B, complete sequence
WM276/2-192                  TAATCCCTTGGGGAGGTTCAGGAACCGTCTGTGGATAAAGATCTGCTGCCGCCAGTCATTAGTCTTTCCATATACCCATCTATCTTTCGTGTGTTTTTTGTCTCTTTTT-CCTCAATGGTAATGATCTCTCTTTGTCAGGTGTTT-TCGGTCAATATTCATTATATAATGATA
//

  • Image source CNB01100, CNAG_03607:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
=GS Found-target/1-13 DE 3'SS intron
Found-target/1-13                  -----------------------------------------------------GTTTCTGTTCTCT--------------------------------------------------------------------------
snRcne03050/1-17                   ------------------------------------------------AGTC-TAAAGGCAAGAGA--------------------------------------------------------------------------
#=GS CNB01100-intron-3'SS/1-138 DE  RING-type domain-containing protein AE017342.1:c334460-334323 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
CNB01100-intron-3'SS/1-138         ACCAAGTGAGTGTTTTACATGCTTAGCATAATGAAGGGCATTAAAGCTGAAC-GTTTCTGTTCTCTAGACAGCGTCTCATCAGGATTTACCGCACTTGATTCCGGGTCTAGCAGTGATT-CATCCTTCATCCTCCTTCTA
#=GS CNAG_03607/1-137 DE CP003821.1:c323863-323727 opposite translation initiation factor aIF-2 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_03607/1-137                   ACCAAGTGAGTGTCTTACATGCTTAGCATAAT-AGGACCATTAAAGCTAAAC-GTTTGTGTTTTGTAGACAGCGTCTCATCAGGATTTACCGCACTTGATTCCGGGTCTAGCAGTGATT-CATCATTCATCCTTCTTGTA
#=GS R265/1-134 DE CP025762.1:1430409-1430542 Cryptococcus gattii VGII R265 chromosome 4
R265/1-134                         ACTAAGTGAGTGTTTATCAT--TTGGCATAAT--GGATCATC-AAGCTGAAC-GTTTTTGTTTCGTAGACAACGTCTCATCAGAATTTACCGCACTTGATTCCAGGTCCAGCAGTAGTTTTACCATTCATCTTTCTTCTA
#=GS WM276/1-134 DE CP000287.1:1471615-1471748 Cryptococcus gattii WM276 chromosome B, complete sequence
WM276/1-134                        ACCAAGTGAGTGTTTACCAT--TCGGCATAAT--AGATCATC-AAGCTGAACTTTTTATGTTTCGTAGACAGCGTCTCATCAGAATTTACCGCACTTGATTCCAGATCCGGCAGTAGTTT-ACCATTCATCTTTCTTCTA
Annot/1-3                          -----------------------------------------------------------------YAG------------------------------------------------------------------------
//

  • Image source CNB02730, CNAG_07481:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
Found-target/1-13              ----------------------------------------------------------------------GTTTTTGTTTTTT-----------------------------------------------------------------------------------
snRcne03050/1-17               -----------------------------------------------------------------AGTC-TAAAGGCAAGAGA-----------------------------------------------------------------------------------
#=GS CNB02730-intron/33-192 DE AE017342.1:815339-815530 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
CNB02730-intron/33-192         GGCTATGTCTTGTGTTTACAAAGGCGGTAGTCAAGATCATGTGGCTACCTGGACATCCGGTATGGCTTTTGTTTTTGTTTTTTTGGCTACTGTTTCCAGAAGAAAAG-TGAAGCTGACGC----CGAA-CAGCTTGTACATCCCTCGGCTTTGATCAGTCTTTTGC
#=GS CNAG_07481/33-188 DE CP003821.1:794872-795059 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_07481/33-188              GGCTATGTCTTGTGTTTACAAAGGCGGTAGTCAAGATCATGTGGCTACCTGGGCATCCAGTATGGCTTTTTTTTTTA---------CTACTGTTTCCAGGGAGAGAACCGAAGCTGACGCAGGCCGAA-CAGCTTGTACATCCCTCAGCCATGATCAGTCTTTTGC
#=GS R265/33-188 DE CP025762.1:c971515-971328 Cryptococcus gattii VGII R265 chromosome 4
R265/33-188                    GGCTGTGTCTTGTGTTTACAAAGGCGGTAGTCAAAATCATGTGGCTACCTGGACATCCAGTACGACTTTTCTTTTT----------CTACTATAGTGTAAGTTATTATAGTGTGAGCTAACGCCAAAAGCAGCTTGTACATCCCTCAGCCTTGATTAGTCTCTTGC
#=GS CryWin/32-95 DE CP034262.1:1326152-1326246 Cryptococcus wingfieldii strain CBS7118 chromosome 2, complete sequence
CryWin/32-95                   GGCTGTGTTTGGTGTTTACCAAAGCGGCAGTCAAGATGATGGGTTTGCCTGGGCATCCTGTATG------------------------------------------------------------------------------------------------------
#=GS CryAmy/33-90 DE XM_019133733.1:963-1052 Cryptococcus amylolentus CBS 6039 hypothetical protein (L202_00542), partial mRNA
CryAmy/33-90                   GGCTGTGTTTGGTGTTTACCAAAGCGGCAGTCAAGATGATGGGTTTGCCTGGGCATCC------------------------------------------------------------------------------------------------------------
#=GS CryFlo/32-95 DE RRZH01000002.1:c952670-952576 Cryptococcus floricola strain DSM 27421 chromosome 2, whole genome shotgun sequence
CryFlo/32-95                   GGCTGTGTTTGGTGTTTACCAAAGCGGCAGTCAAGATGATGGGTTTGCCTGGGCATCCTGTATG------------------------------------------------------------------------------------------------------
#=GS CrySki/32-92 DE BCHT01000011.1:c484007-483916 Cryptococcus skinneri DNA, scaffold: scaffold_10, strain: JCM 9039, whole genome shotgun sequence
CrySki/32-92                   GGTTGTGTTTGGTGTTCACAAAAGCTGTGGTGAAGATCTTCTGGCTACCTGGTCATCCTGT---------------------------------------------------------------------------------------------------------
//

  • Image source CNB03400, CNAG_03850:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
Found-target/1-13             -------------------------------------------------------------------------------GTTTTTGTTCTCT-------------------------------------------------------------------------------------------
snRcne03050/1-17              --------------------------------------------------------------------------AGTC-TAAAGGCAAGAGA-------------------------------------------------------------------------------------------
#=GS CNB03400-intron/1-166 DE  transcriptional activator SPT7 AE017342.1:c1024348-1024183 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
CNB03400-intron/1-166         -TTGCGTAATCAGGCGACTGCCCCCGTTATTCAAGACGATATGATGTTTGCAGATGACGGCGAAATGCTGCTCGAGTCAGTTTTTGTTCTCTCGCTGG--------CTCAA-ATATTAACTT-------TTTTTTTTGCATCAGCGGTAACTTTGCGGATGAGCTCGGAGAGGATTTCCTCGG
#=GS CNAG_03850/1-171 DE  transcriptional activator SPT7 C CP003821.1:c1008618-1008448 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_03850/1-171              -----------AGGCAACTGCTCCCGTCATTCAAGATGATATGATGTTTGCAGACGATGGCGAGATGCTGCTCGAGTCAGTTTCTTTTTCTTTTTTTTTGGTT-GACTCGAGATATTAACTGTCCTTTTTTTTCCTTGCATTAGTGGTAACTTTGCGGATGAGCTCGGAGAGGATTTCCTCGG
#=GS WM276/1-169 DE CP000286.1:582948-583117 Cryptococcus gattii WM276 chromosome A, complete sequence
WM276/1-169                   TTGTCTCCACAAGGCGACTGCTCCTGTTATTCAA-ATGATATGATGTTTGCAGATGACGGCGAGATGCTACTCGAGTGCGTCTTTTTCCTTCTGTCGCTGTTT----TCGG-TTCCTAACT--------TTTTCGCAATTCTAGCGGTAACTTTGCGGATGAGCTCGGGGAAGATTTCCTCGG
#=GS SPT7-exon-a/1-63 DE XM_568915.2:2007-2069 Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNB03400), mRNA
SPT7-exon-a/1-63              ------------GGCGACTGCCCCCGTTATTCAAGACGATATGATGTTTGCAGATGACGGCGAAATGCTGCTCGA------------------------------------------------------------------------------------------------------------
#=GS SPT7-exon-b/1-39 DE XM_568915.2:2070-2108 Cryptococcus neoformans var. neoformans JEC21 hypothetical protein (CNB03400), mRNA
SPT7-exon-b/1-39              ------------------------------------------------------------------------------------------------------------------------------------------------CGGTAACTTTGCGGATGAGCTCGGAGAGGATTTCCTCGG
#=GS CryWin/1-162 DE CP034261.1:1171277-1171439 Cryptococcus wingfieldii strain CBS7118 chromosome 1, complete sequence
CryWin/1-162                  TACACCGCTGTAGACCTCCGCGCCAGTCATTGAAGACGATCAGATGTTTGCGGACGATGGCGAGATGTTGCTAGAGTGAGTTTTTGTGATATTCGAGCGTGG-------CCGAAACTAACACATA--------------TCCAGTGGCAATTTCGCAGAAGAGCTTGGCGAGGATTTCCTTGG
#=GS CryFlo/1-161 DE RRZH01000001.1:c1128425-1128238 Cryptococcus floricola strain DSM 27421 chromosome 1, whole genome shotgun sequence
CryFlo/1-161                  -ATACACCACTGTACCTCCGCGCCAGTCATCGAAGACGATCAGATGTTTGCGGACGATGGCGAGATGTTGCTAGAGTGAGTTTTTGTGATATTCGAGCGTGG-------TCGAAACTAACACATA--------------TCAAGTGGCAATTTCGCAGAAGAGCTTGGCGAGGATTTCCTTGG
#=GS KwoSha/1-173 DE NQVO01000006.1:c241478-241305 Kwoniella shandongensis strain CBS 12478 scaffold00006, whole genome shotgun sequence
KwoSha/1-173                  -TATCCTTGTTTAGCTACTGCACCGGTAATAGGAGATGACATGATGTTCGCAGACAACGGCGAGATGCTGTTAGAGTGAGTGATAACCTATGCCTTCGCTTTCGGAAAACGTAAGCTGATGTCTTGGCTT---------CGTAGTGGAAACTTCGCAGACGAGCTAGGGGAAGATTTCCTCGG
#=GS KocImp/1-151 DE NBSH01000002.1:1011650-1011800 Kockovaella imperatae strain NRRL Y-17943 BD324scaffold_2, whole genome shotgun sequence
KocImp/1-151                  -AGACTGCGGCGC----------CTGTGGTGCATGACGATATGATGTTTGCAGAGGATGGGGAAATGCTGCTCGAGTGAGTGGACGCGATCTGTTGGGTGAAGC--GATAGCTCACGTAG-------------------TGCAGCGGGAACTTTGCCGAAGAGCTGGGAGAGGATTTCCTTGG
#=GS TreFuc/1-160 DE BRDD01000041.1:230943-231125 Tremella fuciformis NBRC 9317 DNA, KCNB80TF.41, whole genome shotgun sequence
TreFuc/1-160                  -CTACAAGGAGGTACGACAGCTCCAGTGATCGAGGACGACATGATGTTCGCAGAGAACGGCGAGATGCTGCTCGAGTGGGTTTCAACGGGTCGGCTGGCGCTGC----ATCACGGCTGACA------------------GACAGTGGCAATTTTGCCGACGAGCTGGGGGAAGATTTCCTTGG
#=GS TreYok/1-159 DE BRDC01000030.1:c527973-527813 Tremella yokohamensis NBRC 100148 DNA, KCNB35TY.30, whole genome shotgun sequence
TreYok/1-159                  -CCTACAAGGAGGACGACAGCTCCAGTGATCGAGGACGACATGATGTTTGCCGAGAACGGCGAGATGCTGCTCGAGTGGGCTTCATCGCGTCGGCTG-CGATGC----ATCATAGCTGACA------------------GACAGTGGCAATTTTGCCGACGAGCTGGGAGAAGATTTCCTTGG
Annot/1-13                    ---------------------------------------------------------------------------GTGrGT----------------------------------CTGA----------------------YAG---------------------------------------
//

  • Image source CNB04310, CNAG_03944:

# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
Found-target/1-13             -----------------------------------------------------------------------------------------------------GTTTCCGTTTTCT------------------------------------------------------------------------------------------------------
snRcne03050/1-17              ------------------------------------------------------------------------------------------------AGTC-TAAAGGCAAGAGA------------------------------------------------------------------------------------------------------
#=GS CNB04310-intron/1-204 DE  chaperone regulator AE017342.1:c1259849-1259646 Cryptococcus neoformans var. neoformans JEC21 chromosome 2 sequence
CNB04310-intron/1-204         CTGCTTTGGCTGGTGGTGACTTTGCCATTGAACACCTTGATGAGCATGCTTTGCACGTCACCATTGTCCCTGGAGAGGTTATCAAGCCTGGTGAGTTT-CAGTTTCCGTTTTCTT---TTTTCACTT-CAAAGAGGATGCG----CACTGACCTTTGTTCCTAACGC---TAGACGCGCTCAAGATTATCTCCGGCCAGGGTATGCCTTCTTACCG
#=GS CNB04310-exon-a/1-90 DE XM_568930.2:931-1020 Cryptococcus neoformans var. neoformans JEC21 chaperone regulator, putative (CNB04310), mRNA
CNB04310-exon-a/1-90          CTGCTTTGGCTGGTGGTGACTTTGCCATTGAACACCTTGATGAGCATGCTTTGCACGTCACCATTGTCCCTGGAGAGGTTATCAAGCCTG------------------------------------------------------------------------------------------------------------------------------
#=GS NB04310-exon-b/2-44 DE XM_568930.2:1020-1063 Cryptococcus neoformans var. neoformans JEC21 chaperone regulator, putative (CNB04310), mRNA
NB04310-exon-b/2-44           -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------ACGCGCTCAAGATTATCTCCGGCCAGGGTATGCCTTCTTACCG
#=GS CNAG_03944/1-205 DE CP003821.1:c1247727-1247523 Cryptococcus neoformans var. grubii H99 chromosome 2, complete sequence
CNAG_03944/1-205              CTGCTTTGGCTGGTGGTGACTTTGCCATTGAGCATCTTGATGAGCATGCTTTGCACGTTACCATTGTCCCTGGAGAGGTTATCAAGCCCGGTGGGTTTTACATTTCCCCTTTCTT---TTTTCATTT-CAAAGGGGATGCA----CACTGACCCTTGTTCCTTACGC---TAGACGCGCTCAAGATTATTTCCGGCCAGGGTATGCCCTCTTACCG
#=GS WM276/1-213 DE CP000286.1:348807-349019 Cryptococcus gattii WM276 chromosome A, complete sequence
WM276/1-213                   CTGCTTTGGCTGGTGGTGACTTCGCCATTGAGCACCTTGACGAGCATGCCTTGCACGTTACCATTGTCCCTGGAGAGGTTATCAAGCCTGGTGAGTTTTTACATTTCCCTTTCTTGTTTTGTCATTTGCAAAGGGAGTGCGAGGGCGCTGACCTTTGTTCCTTACAT---TAGACGCGCTCAAGATTATCTCCGGCCAGGGTATGCCCTCTTACCG
#=GS R265/1-208 DE CP025761.1:c1610850-1610643 Cryptococcus gattii VGII R265 chromosome 3, complete sequence
R265/1-208                    CTGCTTTGGCTGGTGGTGACTTTGCCATTGAGCACCTTGACGAGCATGCCTTGCACGTTACTATTGTCCCTGGAGAGGTTATCAAGCCTGGTGAGTTTTCACATTTTCCTTTCTTGTTTTGTCATTTGCAAAGGGAGTGCGAGGGTGCTGACCCTTGTTCCT--------TAGACGCGCTCAAGATTATCTCCGGCCAGGGTATGCCCTCTTACCG
#=GS CryWin/1-177 DE CP034261.1:c1304641-1304465 Cryptococcus wingfieldii strain CBS7118 chromosome 1, complete sequence
CryWin/1-177                  CTGCTTTGGCTGGTGGTGACTTTGCAATCGAGCACCTCGACGACCGTGCTTTGCACGTCACTATTGTCCCCGGAGAGGTCATCAAGCCTGGTACG-----------TACTCTCGAATTTGTGTG-AGACGTAAG-------------CTGATGATCCT------------CAGATGCTCTCAAGATCATCTCGGGTCAGGGTATGCCCTCTTAC--
#=GS CryAmy/1-131 DE XM_019134842.1:833-963 Cryptococcus amylolentus CBS 6039 hypothetical protein (L202_01440), partial mRNA
CryAmy/1-131                  CTGCTTTGGCTGGTGGTGACTTTGCTATCGAGCACCTCGACGACCGTGCTTTGCACGTCACTATTGTCCCCGGAGAGGTCATCAAGCCTG-----------------------------------------------------------------------------------ATGCTCTCAAGATCATCTCGGGCCAGGGTATGCCCTCTTAC--
#=GS CryFlo/1-177 DE RRZH01000001.1:995679-995855 Cryptococcus floricola strain DSM 27421 chromosome 1, whole genome shotgun sequence
CryFlo/1-177                  CTGCTTTGGCTGGTGGTGACTTTGCAATCGAGCACCTCGACGACCGTGCTTTGCACGTTACTATTGTCCCCGGAGAGGTCATCAAGCCTGGTAAG-----------TACTCTCGAATTTGTGTG-AGACGTAAG-------------CTCATGATTCG------------CAGATGCTCTCAAGATCATCTCGGGCCAGGGTATGCCCTCTTAC--
#=GS KwoSha/3-207 DE NQVO01000014.1:c467348-467140 Kwoniella shandongensis strain CBS 12478 scaffold00014, whole genome shotgun sequence
KwoSha/3-207                  CAGCTTTGGGTGGTGGTGAATTCGCTATCGAGCACTTGGATGAGCGAGCATTGCACGTTACCATTGTTCCTGGAGAGATCATCAAGCCTGGTACGTTTGCGCTCATAACAGAATGACATGTGTGTAGATGGCAGTG-----------CTAACATTGTATCACATTCACTACAGGCGCCCTCAAGGTCATCTCCGGCCAAGGTATGCCCTCTTACCG
Annot/1-13                    ------------------------------------------------------------------------------------------GTGrGT---------------------------------------------------CTGA-------------------YAG-------------------------------------------
//