Loci with complementarity to snRcnag12093

Target	Found @		Possible mRNA target [1]			Genes
	JEC21	H99	Orientation	Overlap	Conserved	JEC21	H99	Gene-product
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TAGTAGCCTTTTGGTTGA	1:663438-663455:1		as-guide	3’UTR exon	no	CNA02535		Serine/threonine/tyrosine-interacting protein
TGGCAGCCTTCTGGTTG	1:2034497-2034513:-1		as-guide	CDS exon	no	CNA07420	CNAG_00764	DBP4
TGGTAGCCTTCTGGTTG	4:947359:947373:1	5:874268-874284:-1	as-guide	5’SS CDS-exon	yes	CND03490	CNAG_01230	Chitin deacetylase 2 · Gene: CDA2
TGGCAGCCTTCTGGTT	5:956253:956268:1		intergenic
TGATGGTTTTTTGGT	1:1095638-1095652:-1	1:1104216-1104230:-1	as-guide	CDS-exon	no	CNA04080	CNAG_00422	DNA replication regulator SLD2
TAACGGTCTTTTGGT	1:2060712-2060726:-1	1:2034667-2034681:-1	as-guide	CDS-exon		CNA07530	CNAG_00775	UTP15, U3 small nucleolar RNA-associated protein 15
TGGCAGTTTTCTAG	1:91096-91109:1		as-guide	opp 3’SS
TAATGGTCTTTTAG	1:240729-240742:-1	1:262727-262741:-1	target	3’end	no	CNA00860	CNAG_00095	RNA polymerase II assembly factor Rtp1 C-terminal domain-containing protein
TAACAGCCTTCTGG	1:994738-994751:-1		intergenic
TGATAGCCTTTTGG	1:1605503-1605516:1		as-guide	CDS-exon		CNA06000	CNAG_07373	Carbamoyl-phosphate synthase, large subunit
TGATAGCTTTCTGG	1:1663462-1663475:1		as-guide	CDS-exon		CNA06150	CNAG_00635	Cytoplasmic protein
TGACGGCCTTCTAG	3:327495-327508:1		as-guide	CDS-exon		CNC01170	CNAG_01604	HAD hydrolase
TGGCGGTCTTCTGG	3:742767-742780:1	11:809968-809982:1	target	CDS-exon	no	CNC02600	CNAG_01759	Vacuolar membrane protein
TGGCAGTCTTTTGG	3:1787183-1787196:-1	3:245252-245266:1	target	last CDS-exon	no	CNC06080	CNAG_02998	Nuclear protein
TGGCGGTCTTTTAG	4:512145-512158:-1		target	CDS-exon	no	CND01900	CNAG_01059	Vacuolar membrane protein
TGGCAGCCTTTTGG	4:861790-861803:-1		intergenic
TGATGGCTTTCTGG	4:947145-947158:1		as-guide	CDS-intron		CND03490	CNAG_01230	Chitin deacetylase 2 · Gene: CDA2
TAACAGTCTTCTAG	4:1065423-1065436:-1		as-guide	3’UTR exon		CND03840	CNAG_01263	Vacuole fusion, non-autophagic-related protein
TAGCAGCTTTTTGG	5:1288113-1288126:1		target	CDS-exon	no	CNE04600	CNAG_02046	Leukotriene-A4 hydrolase
TGACAGTCTTCTAG	6:190592-190605:-1	7:1215722-1215736:1	target	3’SS-intron	no	CNF00560	CNAG_05919	Nitrogen permease regulator 3
TGATGGCTTTCTGG	6:746157-746170:1		target	intron	no	CNF02580	CNAG_05716	Essential protein Yae1 N-terminal domain-containing protein
TAGCAGCTTTTTGG	6:1315860-1315873:1		as-guide	CDS-exon		CNF04530
TGACAGTCTTCTGG	6:1320895-1320908:-1		as-guide	CDS-exon		CNF04540
TGGTAGTCTTCTGG	6:1390769-1390782:-1		as-guide	CDS-exon		CNF04770
TAACGGTTTTTTAG	7:423469-423482:-1		as-guide	intron		CNG01470
TGATAGCTTTCTGG	7:796991-797004:1		as-guide	CDS-exon		CNG02860
TAATAGTCTTCTAG	7:969413-969426:-1		as-guide	CDS-exon		CNG03450		transposon ? (RNAi suppressed in JEC21)
TGGTAGTCTTTTGG	8:191090-191103:-1		as-guide	CDS-exon		8_use_w-Cnl1_743
TAATAGCTTTCTGG	8:244443-244456:1		target	CDS-exon	no	CNH03060	CNAG_00867	Myo-inositol transporter (one of a few)
TAACGGTCTTTTGG	8:480334-480344:1		as-guide	CDS-exon		CNH02330
TGACAGTCTTTTGG	8:457521-457534:1		intergenic
TGGCAGCTTTTTGG	9:1082673-1082686:1		as-guide	CDS-exon		CNI04070
TAATAGCTTTCTGG	10:1075824-1075837:1		target	CDS-exon 1	no	CNJ03420 (as CNH03060)
TAATAGTCTTTTGG	11:128813-128826:1		as-guide	intron/intergenic		CNK00430
TGACAGTCTTTTAG	11:784311-784324:-1		target	3’UTR-exon	no	CNK02700	CNAG_01930	Endopeptidase (RNAi suppressed in JEC21)
TGGTGGCTTTCTGG	12:237408-237421:-1		target	intron	no	CNL04450	CNAG_05181	White collar 1 protein
TGACAGTCTTTTAG	13:332314-132327:-1		target	CDS-exon	no	CNM01110	CNAG_06104	Beta-glucuronidase C-terminal domain-containing protein
TGACAGTCTTCTGG	14:205257-205264:1		target	intron	no	CNN00630	CNAG_06333	Replication factor C subunit 3/5
-rryRgyyuuyurguugr
GGCGGCtTTCTGGTTGA	1:582841-582847:-1	1:612299-612316:-1	target	intron	no	CNA02220	CNAG_00232	Large subunit ribosomal protein L30e (L32) [2]
AACAGCCTTCTAGTTGA	1:1859614-1859630:-1		as-guide	5’UTR		cnag120093		transcript with intronic snoRNA gene
AATGGTCTTTTGGTTGG	AATGGTCTTTTGGTTG-	1:819412-819428:-1	as-guide	CDS exon		CNA03050	CNAG_00320	Anaphase-promoting complex subunit 3
GACGGTCTTCTGGTTG	1:1408774-1408789:-1		as-guide	CDS exon		CNA05300
AATGGCCTTTTGGTTG	6:347805-347820:1		as-guide	3’UTR		CNF01130
AGCAGCTTTCTGGTT	1:2040504-2040518:1		as-guide	CDS-exon		CNA07450
AATGGTTTTCTAGTT	2:1596957-1596971:1	AATGGTTTTCTAG-	target	3’SS intron	no	CNB05690	CNAG_04090	bZip transcription factor
AGCAGCCTTTTGGTT	3:932189-932203:1	AGTcGCCTTTTccTT	target	intron	no	CNC02870	CNAG_01796	Uncharacterized protein
GGTAGCCTTCTGGTT	4:947358-947372:1		as-guide	CDS-exon		CND03490	CNAG_01230	Chitin deacetylase 2 · Gene: CDA2
AATGGCCTTTTGGTT	6:347805-347819:1		as-guide	5’UTR-exon		CNF01130
AACAGCCTTCTAGTT	12:814651-814665:-1		target	CDS-exon	no	CNL06520	CNAG_04963	Asl1-like glycosyl hydrolase catalytic domain-containing protein
AGCAGCTTTTTGGT	1:391086-391099:1		as-guide	3’UTR-exon		CNA01450
AGCAGCTTTCTGGT	1:1802066-1802079:-1		as-guide	CDS-exon		CNA06610
GGCAGCTTTTTGGT	1:2244776-2244789:-1		target	5’ transcript	no	CNA08150	CNAG_00838	YCII-related domain-containing protein
AATGGTCTTTTGGT	2:303756-303769:-1		target	CDS-exon	no	CNB01000	CNAG_03597	CSC1/OSCA1-like 7TM-domain-containing protein
AGTAGTTTTTTGGT	2:909545-909558:1		target?	transcript	no	opposite CNB02995		No annotation, transcript observed, from centromeric region
GGCGGTTTTCTAGT	2:127635-127648:-1		intergenic
AACGGTCTTTTGGT	2:1618498-1618511:-1		as-guide	CDS-exon		CNB05770
	3:1801872-1801885:1		target	CDS-exon	no	CNC06150	CNAG_03004	riboflavin synthase, alpha subunit
GATAGTCTTTTGGT	3:580340-580353:-1		target	CDS-exon	no	CNC02070	CNAG_07598	importin-alpha export receptor
GACGGTCTTTTGGT	4:24302-24315:-1		as-guide	CDS-exon		CND00090
GACAGCCTTTTGGT	5:265274-265287:1		as-guide	CDS-exon		CNE00960
AGCGGCTTTTTGGT	5:1089892-1089905:-1		as-guide	CDS-exon		CNE03810
AATGGTCTTCTGGT	6:102360-102373:1		as-guide	CDS-exon		CNF00300
GGTGGCTTTTTAGT	7:783429-783442:1		as-guide	3’UTR exon		CNG02825
AGTGGTCTTCTAGT	7:932676-932689:1		target ? or intergenic (as opposite CNB02995)			opposite CNG03300-3310
AGCGGTCTTTTGGT	8:470585-470598:1		target	CDS-exon	no	CNH02350	CNAG_05590	protein-histidine kinase
AGCGGTTTTTTAGT	8:817792-817805:1	AGCGGTTTgcTAGg	target	3’UTR exon	no	CNH01277	CNAG_05467	Uncharacterized protein
AACAGCTTTTTAGT	8:1091424-1091437:1		intergenic
AATGGTCTTCTAGT	9:659208-659221:1		intergenic
GACAGCTTTTTGGT	9:980329-980342:-1		as-guide	CDS-exon		CNI03640
AACGGCCTTTTGGT	10:159399-159412:-1		as-guide	CDS-exon		CNJ00570
AGCAGTTTTCTGGT	12:199052-199065:-1	AGCAaTTTTCTGGT	target	CDS-exon	no	CNL04310	CNAG_07641	monosaccharide transporter
GGCAGCTTTCTGGT	14:56896-56909:-1		target	CDS-exon	no	CNN00150	CNAG_06259	alpha-glucoside:hydrogen symporter

[1]

Orientation ‘target’ implies described RNA could base-pair to snRcnag12093 guide; ‘as-guide’ means that search sequence is antisense to described RNA, thus like the complementary snRcnag12093 guide. In this case no further analysis was done. ‘Conserved’ describes occurrence of target at same location in homologous genes in species beyond Cryptococcus that could express snRcnag12093 homologous snoRNAs (Tremellomycetes).

[2]

Could lead to retained intron in H99/JEC21 by blocking splicing through basepairing upstrean BP (CTGA)