CNK02420, annotated as a pseudo-gene, produces a transcript with two intronic box CD snoRNAs, as CNAG_13018
The two box CD snoRNAs appear to be specific for Cryptococcus
Conserved regions could form accessory base pair interactions with (pre-)rRNA or contribute to a secondary structure
>AE017351.1:c703687-702986 Cryptococcus neoformans var. neoformans JEC21 chromosome 11 sequence CACCTGCGTCAACTATTTCCTGCACTGCTGTTGTGCTTCTTCTCTTTCATCACCCAGTCCTTGCCAACTC AGCTCATCGGGATCCCGAAGACCGCAGTTCGACCTTGTTGCCTCAACAGTAACAATAACTGAGACAATAA TTGGCTAACAAGTGAGTTCAATGTTCTTTTTCCTCCTTGACCTTTTCTTGCCTTCAATGATGTCTTAAGC ATGTATTGTCAGACCTTGGGGTAGCACCCATTGTCGAATGTTGATCAAATATAGTCAGAATCGCCCTGAT TCCCATTTCTATCCCCTTTTGACTATGGTTTAGATATTGGAAGTAGGAGCTGATGTTGGATCCACAGGTT TGAACAGTTTGCTACTAGTAGCGCTTGGCGTGTGATTTATTATTGAAAAGTAAGTCGGTTACACTATTTG ATCTACCCCTTCCCTTTTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAG AGAACTGGTTAGAGCCGTTCTGATCCTTTTATATCCTTCATTTGTGTTTATTAGAAGTAAGACCATATGC TGACTGCGTATATCTAGGTTTAGCAAAGTAAGTCTGAAACACCTTCATTCAAGCTATTTTTGTCTTCCAT TCTCGTCTTTGTACCCGTATTGTATCCAGTTGCTATATTGCCGACCACTTCATGTATTTTGTTTCGTATC AA
snRcnk02420-1¶
snRcnk02420-1_intronic-boxCDsnoRNA-89nt-in-CNK02420pseu-as-CNAG_13018
Within 1st intron of CNK02420 (CNAG_13018)
Possible target D’ guide in 18S rRNA: taaTacatgct
Possible target D guide in 25S rRNA: tgAttttgatt
No orthologues found
Alternative targets with full complimentarity to the D-guide are listed here and shown below
>AE017351.1:c703495-703407 Cryptococcus neoformans var. neoformans JEC21 chromosome 11 sequence TTCAATGATGTCTTAAGCATGTATTGTCAGACCTTGGGGTAGCACCCATTGTCGAATGTTGATCAAATAT AGTCAGAATCGCCCTGATT
Alternative targets D-guide snRcnk02420-1
Only candidate targets with potential to base-pair to the full guide (and one with a mnismatch):
CNG03750 (CGFDS motif), CNAG_03194 just upstream 5’ splice site¶ CNG04660 (AILIL motif), CNAG_03099¶Tremellomycetes targeted segments
snRcnk02420-2¶
snRcnk02420-2_intronic-boxCDsnoRNA-82nt-in-CNK02420pseu-as-CNAG_13018
Within 2nd intron of CNK02420 (CNAG_13018)
Possible target D’ guide in 18S rRNA: aagCcatgcatgt
Accessory interaction via actggttagagcc upstream of the D-box can occur with ggctttggctggt in 18S rRNA
No orthologues found
Alternative targets for the D-guide are listed in here and shown below
>AE017351.1:c703252-703171 Cryptococcus neoformans var. neoformans JEC21 chromosome 11 sequence TTTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGC CGTTCTGATCCT
Alternative targets D-guide snRcnk02420-2
Only candidate targets with potential to base pair to the full guide:
CNL06030 (NGFD motif), CNAG_05013¶Tremellomycetes targeted segments
Image source CNK02420, CNAG_13018:
# STOCKHOLM 1.0
#=GF https ://coalispr.codeberg.page/paper
#=GF RW van Nues, July 2023
Annot/1-118 -----------------------------------------------------------------------------------------------------------------------------------------------------------------GTGrGT----------------------------------------RTGATGA----UCGUACAUAAU-CTGA-------------------------RTGATGA---------UUAGUUUUAGU--CTGA--------------------------------------------------CTGA----------------YAG-----------------------------------------------------------------------------------------------------------------------------GTGrGT-------------------------------------------RTGATGA-----------UGUACGUACCGAA-CTGA--------RTGATGA--UGGUCGGUUUCGG---CTGA----------------------------------------------CTGA-----------YAG----------------------------------------------------------------------------------------------------------------------------------------------------
#=GS CNK02420-pseu/1-702 DE spliced-transcript-with-2-boxcd-snoRNAs-as-CNAG_13018
CNK02420-pseu/1-702 CACCTGCGTCAACTATTTC--CTGCACTGCTGTTGTGCTTCTTCTCTTTCATCACCCAGTCCTTGCCAACTCAGCTCATCGGGATCCCGAAGACC----GCAGTTCGACCTTGTTGCCTCAA----CAGTAACAATAACTGAGACAATAATTGGCTAACAAGTGAGTTCAATGTTCTTTTTCCTCCTTGACCTTTTCTTGCC-TTCAATGATGTCTTAAGCATGTATTGTCAGACCTTGGGGTAGCA-CCCATTGTCGAATGTTGATC-AAATATAGTCAGAATCGCCCTGATTCCCATTTCTATCCCCTTTTGACTATGGTTTAGATATTGGAAGTAGGAGCTGATGTTGGAT-----CCACAGGTTTGAACA-GTTTGCTACTAGTAGCGCTTGGCG-----------------------------------------------------------------------TGTGATTTATTATTGAAA-AGTAAGTCGGTTACACTATTTGATCTACC--------CCTTCCCTTTTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATCCTTTTATATCCTTCATTTGTGTTTATTAGAAGTAAGACCATATGCTGACTGCGTATATCTAGGTTTA-----GCAAAGTAAGTCTGAAACACCTTCATTCAAGCTATTTT------------------TGTCTTCCATTCTCGTCTTTGTACCCGTATTGTATCCAGTTGCTATATTGCCGACCACTTCATGTATTTTGTTTCGTATCAA
#=GS CNAG_13018-a/1-392 DE CP003830.1:c1216731-1216340 Cryptococcus neoformans var. grubii H99 chromosome 11, complete sequence
CNAG_13018-a/1-392 CAACTGCGTCGACTACGTATACTGCACTGTTCTTGTGTTTTTTCTCTTTTATCGTTCAGTACTTGCCAAGGCAGCTCATCGGGATCCCGGAGACCCGACGCAGTTCGATCTCGTTGCCTCACTCAACAGCAACAATAATTGAGACAATAGTTGGCTAACAAGTGAGTTCAATGTTCTTTTTCTTCCTTCACTTTTTCTTGCCTTCAAATGATGCCTTAAGCATGTATTGTCAGACCTTGGGGTAGCA-CCCATTGTCGAATGTTGATCAAAATATAGTCAGAATCGCCCTGATTCC-ATTTCTATCCCGTTTTAACTATGGTTTAGTTATCCGAAGTATGAGCTGATGTTGGAT-----CCACAGGTTTGAACAAGTTTGGTATTAGTAGCGCTTGGCG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GS CNAG_13018-b/1-329 DE CP003830.1:c1216267-1215939 Cryptococcus neoformans var. grubii H99 chromosome 11, complete sequence
CNAG_13018-b/1-329 --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TGTAATTTATCATTGAAATAGTAAGTCGGTTACACTATTTGACCTATC--------CATTTCCTTTTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATCCTTCTATATCCTTTACTTGTGTTTATTAGAAGTAAGGCCATATGCTGACTGCGTATGTTTAGGTTTATTTAACACCAGTAAGTCTGAAACACCTTCATTCAAGCTATTTTT-----------------TGTCTTCCATTCTCGTCTTTGTACCCGTATTGTATTCAGTTGCTACATCGCCAACCATTCCATGTATTTTATTTCATACCAA
#=GS snoRNAs-from-CNAG_13018/1-171 DE 11: [1,216,438 - 1,216,528] (+90) - snoRNA2 11: [1,216,131 - 1,216,210] (+79)
snoRNAs-from-CNAG_13018/1-171 -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TCAAATGATGCCTTAAGCATGTATTGTCAGACCTTGGGGTAGCA-CCCATTGTCGAATGTTGATCAAAATATAGTCAGAATCGCCCTGATT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GS snRcnk02420-1_-2/1-172 DE intronic-boxCDsnoRNAs-in-CNK02420pseu-as-CNAG_0113018
snRcnk02420-1_-2/1-172 -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTCAATGATGTCTTAAGCATGTATTGTCAGACCTTGGGGTAGCA-CCCATTGTCGAATGTTGATC-AAATATAGTCAGAATCGCCCTGATT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TTTCATGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATCCT----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GS R265/1-787 DE CP025759.1:318963-319749 Cryptococcus gattii VGII R265 chromosome 1, complete sequence
R265/1-787 -CTGTATTGTTCCACTGCTTGCAACACTTCGCTTGTGCTTTTTCTCTTCCATCGGTCAGAACTTGCCAACTCAGCTCATAGAGAT-TCGAAGATCACT----ATTTGACCTTGTTGCTTAGTTCAACCACAATAATAATCAAGACAACAATTGGTTAGCAGGTGAGTTTCATATTCTTTACCCTC-CTTCACCTTTCCTTGCTTTCAATGATGCCTTAAGCATGTATTGTCAGACTTTGGGGTAGCA-CCTATCGTCGAATGTTGAT--AAATATAGTCAGAATCGCCCTGATGCCCATGTCCATCCCCTTTGGATTTTGGTTTAGTTATCCGAGGTATGAGCTGATGTCGGAG-----CCACAGGTTTGAATA-GTTTGATATTAGTTGCAGTTGTCCAGAGCCATTTCCCGTGATATTCGAGGAATCTTCAGCGTAACAACGGCCCGATATCGCTGTAAAATCCGTTTTATAGTCCACCATTGAA-CAGTAAGTCGGTTATACCATTTGACCTATC-----TCCCACTCCCTTTTTCGTGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATCTTTTTATATCCTTTACTTGTGTTTATTAGGAGTAAGGCCATATGCTGACTGCGAATGTCTAGGTTTA-----GCAAAGTAAGTCTGAAACACCTCAATTCAAATACTTTTATTCCAG--GTTTTTTTATGTCTTCCATGCATGTCTTTGTACCCGTATTGCACTCAGATGCTGTATTGCCTAGCA-TCCATGTACTTTGTTTC-------
#=GS WM276/1-774 DE CP000296.1:316168-316941 Cryptococcus gattii WM276 chromosome K, complete sequence
WM276/1-774 --TGTCTTGTTCAACTACTTGCAACGCTTCGCTTGTGCTTTTTCTCTTCCATCGCTCAGAACTTGCCAACTCAGCTCATAGATATATCGAATATCACT----ATTTGACCTTGTTGCCTTGTTCAACAACGACAATAATCAAGGCAACAATTGGCTAGCAGGTGAGTTTCATGTTCTTTACCCTCACTTCACCTTTTCTTGCTTTCAATGATGCCTTAAGCATGTATTGTCAGACTTTGGGGTAGCA-CCTATCGTCGAATGTTGAT--AAATATAGTCAGAATCGCCCTGATGCCCTTGTCCATCTCCTTTGGATTTTGGTTTAGTTAGCCGACATATGAGCTGATGTCAGAT-----CCACAGGTTCGAATA-GTTTGATATTAGTTGCACTTGGCCAGACCCATTTCC---------------ATTTTCAGCGTAGCAACGGCCTGTTATCGCTGCAAGTCCCGTTTTATGGTCTATCATTGAA-CAGTAAGTCGGTTACACCATTTGACCTATC------CCCACTCCCTTTTTCGTGATGTTACTCTTTTAAATATGCATGGCTTTCAGAAAACCTGCATGTAGAGAACTGGTTAGAGCCGTTCTGATCTTTTTGTATCCTTTACTTGTGTTTATTTGAAGTAAGGCCATACGCTGACTGCGAATGTCTAGGTTTA-----GCAAAGTGAGTCTGAAATACCTCAATTCAAATACTTTTATTACAACCTTTTTTTTTTGTCTTCCATGCACGTCTTTGTACTCGTATTGCACTCAGCTGCTGTATTGCCTAGCA-TCCATGTACTTTGTTTC-------
#=GS CryAmy/1-364 DE MEKH01000014.1:c367639-367276 Cryptococcus amylolentus CBS 6273 supercont2.14, whole genome shotgun sequence
CryAmy/1-364 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTTGAAGGATGCC--AAGCATGTATTGTCCGACCTTGGCGTGATAACCAA--GTCGAATGTTGAATCAACCATAGTCAGAATCGCACTGATACTCTTACCTCTTACTTCGTTGAATTGTGTCGAGGAAGGGTCAT-TGTGCTGACACTT--ATTTCCTTCTAGTCTTCATT--TTGGAACACAATCAGCAATTCGCTTCGCACAGA-CCC-------TCGACTTTGCCCC--------TTTGGACGCAGACAGTT-AAAGTAGC------------------GAATCAGTGAGTCATCTTCGCCAAT-GATCTTTCCACCATCCTCCATCCTCTTCCCTGATGACAACCT-TTATATATGCATGGCTTTCAGAA-CTTTGC-TGTAGAGAACTGGTTAGAACCTTTCTGATC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GS CryWin/1-372 DE AWGH01000006.1:c348739-348368 Cryptococcus wingfieldii CBS 7118 supercont1.6, whole genome shotgun sequence
CryWin/1-372 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTTCAAGGATGTT--AAGCATGTATTGTCAGACCTTGGCGTGATAACCAA--GTCGAATGTTGAATCAACCATAGTCAGAATCGCACTGATACTTTTACCTCTTGCTTCGTTAAATCGTGTCGAGGAAGGATCAT-TGTGCTGAC-CTTTTTTTTCCTTCTAGGCTTCATC--TTGGAACACGATCAGCAATTCGCTTCGCACAGAACCC-------TCGACTTTACCCCC--------TTGGACGCAGACAGTTGAAAATAGC------------------GAATCAGTGAGTCATCTCCGCCATT-GATCTTTCCACCATCCACCATCCTCTTCCCTGATGACAACCT-TTATATATGCATGGCTTTCAGAA-CTTTGC-TGTAGAGAACTGGTTAGAACCTTTCTGATCCATTT-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GS CryFlo/1-368 DE RRZH01000010.1:c410133-409766 Cryptococcus floricola strain DSM 27421 chromosome 9, whole genome shotgun sequence
CryFlo/1-368 ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------CTTCAAGGATGTT--ACGCATGTATTGTCAGACCTTGGCGTGATAACCAA--GTCGAATGTCGAATCAACCATAGTCAGAATCGCACTGATACTTTGACCTCTTGCTTTGTTAAATTGTGTCGATGAAGGATCAT-TGTGCTGACCTTTTTTTTTCCTTCTAGGCTTCATT--TTGGAACACGATCAGCAATTCGCTTCGCACAGAACCC-------TCGACTTTGCCCCCC--------TGGACGCAGACAGTTGAAAATAGC------------------GAATCAGTGAGTCATCTCGCCCATT-GATCTTTCCACCATCCTCCATCCTCTTCCCTGATGACAACCT-TTATATATGCATGGCTTTCAGAA-CCTTGC-TGTAGAGAACTGGTTAGAACCTTTCTGATC------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
#=GC SS_cons --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------((((--------))))---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------((..((((..)))).))------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
//
Image source snRcnk02420-1D target CNG03750, CNAG_03194:
# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
snRcnk02420-1-D/1-20 -------------------------------------agtc-cCGCTAAGACTGATAT------------------------------------------------------------
Target-found/1-15 -------------------------------------------GTGGTTTTGATTCTG-------GTGrGT-----------------------------------------------
#=GS CNG03750/1-116 DE AE017347.1:c1052476-1052361 Cryptococcus neoformans var. neoformans JEC21 chromosome 7 sequence
CNG03750/1-116 CATTCGATCGCCAGCTCCACGGGGGCCTGTATTGTTCCTTCCTGTGGTTTTGATTCTGTTCCTTCGTGAGCAACTTTA-TCTTAGTTGATTGGAATCAAGCTGATTGGTTGATCTAA-
#=GS CNAG_03194/1-116 DE saccharopine dehydrogenase CP003827.1:311935-312050 Cryptococcus neoformans var. grubii H99 chromosome 8, complete sequence
CNAG_03194/1-116 CATTCGATCGCCAGCTCTACGGGAGCCTGTATTGTTCCTTCCTGTGGTTTTGATTCTGTCCCTTCGTGAGCAACTTCA-TCTCAGTTGGTTGGAATCAAACTGATAGGCTTATCTAA-
#=GS R265/1-117 DE CP025765.1:272128-272244 Cryptococcus gattii VGII R265 chromosome 7, complete sequence
R265/1-117 CATTCGATCGCCAGCTCGACTGGGGCCTGCATTGTTCCTTCCTGCGGTTTTGATTCTGTTCCTTCGTGAGCGACTTTA-TCTTCGTCGGTTGAAATCAAGCTGATTTAGTTGATCCAA
#=GS WM276/1-115 DE CP000292.1:285138-285252 Cryptococcus gattii WM276 chromosome G, complete sequence
WM276/1-115 CATTCGATCGCCAGCTCGACTGGGGCCTGCATTGTTCCTTCCTGCGGTTTTGATTCTGTTCCTTCGTGAGCGACTTCAATCTTAATCGGTTGAAATCAAACTGAATTGGTTGATC---
#=GS CryAmy/1-52 DE MEKH01000007.1:262966-263017 Cryptococcus amylolentus CBS 6273 supercont2.7, whole genome shotgun sequence
CryAmy/1-52 ------------------ACTGGAGCATGCATCGTGCCTGCTAGTGGATTTGACTCTGTCCCTTCGTAAG------------------------------------------------
#=GS CryWin/1-52 DE AWGH01000013.1:343416-343467 Cryptococcus wingfieldii CBS 7118 supercont1.13, whole genome shotgun sequence
CryWin/1-52 ------------------ACTGGAGCTTGCATCGTGCCTGCTAGTGGATTTGACTCTGTCCCTTCGTAAG------------------------------------------------
#=GS CrySki/1-52 DE BCHT01000002.1:2192630-2192681 Cryptococcus skinneri DNA, scaffold: scaffold_1, strain: JCM 9039, whole genome shotgun sequence
CrySki/1-52 ------------------ACTGGAGCATGTATCGTCCCAAGTTGTGGGTTTGATGCGGTGCCCTCGTGAG------------------------------------------------
#=GS CryFlo/1-28 DE RRZH01000007.1:c312910-312883 Cryptococcus floricola strain DSM 27421 chromosome 7 DSM27421_contig_07, whole genome shotgun sequence
CryFlo/1-28 -------------------------------------------GTGGATTCGACTCTGTCCCTTCGTAAGC-----------------------------------------------
#=GS KwoHev/1-61 DE ASQC01000029.1:182177-182237 Kwoniella heveanensis CBS 569 cont2.29, whole genome shotgun sequence
KwoHev/1-61 ---------GCTAGCTCGACGGGAGCTTGCATAGTGCCTTCGTGCGGCTTTGACTCTGTGCCCTCGTGAG------------------------------------------------
#=GS TreTag/1-63 DE CAJHEQ010000421.1:12842-12904 MAG: Tremellales sp. Tagirdzhanova-0007 genome assembly, contig: TREM_421, whole genome shotgun sequence
TreTag/1-63 -------TCGCTAGCAAAACTGGTGCATGTATAGTCCCAAGCTGTGGTTTCGACTCTGTTCCGTCGTGAG------------------------------------------------
#=GS CryGC4/1-70 DE JALPCA010000056.1:24248-24317 Cryptococcus sp. GC_Crypt_4 iso00_46_85, whole genome shotgun sequence
CryGC4/1-70 ---------GCCAGCAAGACCGGTGCATGTATTGTCCCAAGCTGTGGATTCGACTCTATACCATCGTGAGCAGTACTTT---------------------------------------
//
Image source snRcnk02420-1D target CNG04660, CNAG_03099:
# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
snRcnk02420-2-D/1-20 ----------------------------------------------------agtc-cCGCTAAGACTGATAT---------------------------------------------
Target-found/1-15 ----------------------------------------------------------GCGATTTTGATTTTG---------------------------------------------
#=GS CNG04660/1-118 DE AE017347.1:1331684-1331801 Cryptococcus neoformans var. neoformans JEC21 chromosome 7 sequence
CNG04660/1-118 GAGATCGTTTGAAGAGGCGATCCCGCTGATGTTGTTGGCGATCGTGTTGGGCTTGCCGGCGATTTTGATTTTGATTACGACGAGAAAAGTGGTGTATGTGGCATGGATGCTGGTTTAT
#=GS CNAG_03099/1-118 DE chitin synthase 1 CP003827.1:c46279-46162 Cryptococcus neoformans var. grubii H99 chromosome 8, complete sequence
CNAG_03099/1-118 GAAATCGTTTGAAGAGGCGATCCCGCTGATGTTGTTGGCGATCGTGTTGGGCTTGCCGGCGATTTTGATTTTGATCACGACGAGAAAGGTGGTGTATGTGGCATGGATGCTGGTTTAT
#=GS R265/1-118 DE CP025765.1:c6787-6670 Cryptococcus gattii VGII R265 chromosome 7, complete sequence
R265/1-118 GAAATCATTTGAAGAAGCGATCCCGTTGATGTTGTTGGCGATCGTGTTGGGCTTGCCGGCGATTTTGATTTTGATCACAACAAGAAAAGCGGTATACGTGGCATGGATGCTGGTTTAT
#=GS WM276/1-118 DE CP000292.1:c8208-8091 Cryptococcus gattii WM276 chromosome G, complete sequence
WM276/1-118 GAAATCATTTGAAGAAGCGATCCCGTTGATGTTGTTGGCGATCGTGTTGGGCTTGCCGGCGATTTTGATTTTGATCACAACAAGGAAAGTGGTATACGTGGCATGGATGCTGGTTTAT
#=GS CryWin/1-111 DE CP034267.1:4341-4451 Cryptococcus wingfieldii strain CBS7118 chromosome 7, complete sequence
CryWin/1-111 -------TTTGAAGAGGCGATCCCGTTGTTGCTGTTGGCGGTCGTATTGGGTCTGCCGGCGATTTTGATATTGATCACGACGCGAAAGGTGATTTATGTGGCTTGGATGTTGGTGTAT
#=GS CryAmy/1-111 DE MEKH01000016.1:3515-3625 Cryptococcus amylolentus CBS 6273 supercont2.16, whole genome shotgun sequence
CryAmy/1-111 -------TTCGAAGAGGCGATCCCGCTGTTGTTGTTGGCGGTTGTATTGGGTCTGCCGGCGATTTTGATCTTGATCACGACGAGAAAGGTGATTTATGTGGCTTGGATGTTGGTGTAT
#=GS CryFlo/1-111 DE RRZH01000007.1:13277-13387 Cryptococcus floricola strain DSM 27421 chromosome 7 DSM27421_contig_07, whole genome shotgun sequence
CryFlo/1-111 -------TTCGAAGAGGCGATTCCGCTGTTGTTGTTGGCGGTTGTATTGGGTCTGCCGGCGATCTTGATATTGATCACGACGAGAAAGGTGATTTATGTGGCTTGGATGCTGGTGTAT
#=GS KwoSha/1-106 DE NQVO01000019.1:145757-145862 Kwoniella shandongensis strain CBS 12478 scaffold00019, whole genome shotgun sequence
KwoSha/1-106 ---ATCGTTCCAAGAGGCCATTCCTCTGATGTTGTTGGCGATCGTTTTGGGTTTACCAGCGATCTTGATCTTGATCACGACTCGTAAGGTGATCTATGTCGCTTGGATG---------
#=GS PapLau/1-116 DE JAAZPW010000006.1:794789-794904 Papiliotrema laurentii strain IF7SW-B5 scaffold13_cov182, whole genome shotgun sequence
PapLau/1-116 -AGCTCGTTCCAAGAGGCGATTCCGTTGATGCTCCTCGCTGCGGTGTTGGGGCTGCCCGCGGTGTTGATCCTCATCACGACGAGAAAGGTGATCTACGTGGCGTGGATGGTGGTGTA-
#=GS CrySki/1-107 DE BCHT01000002.1:1923268-1923374 Cryptococcus skinneri DNA, scaffold: scaffold_1, strain: JCM 9039, whole genome shotgun sequence
CrySki/1-107 ----TCATTCGAGGAGGCTATTCCGCTTATGCTGCTTGGGGCAGTGCTCGGTCTACCAGCGTTGTTAATTTTGATTACAACGAGAAAAGTCATCTATGTGGCGTGGATGCT-------
#=GS TreMes/1-115 DE AFVY01000363.1:c56213-56099 Tremella mesenterica DSM 1558 strain Fries TREMEscaffold_16_Cont363, whole genome shotgun sequence
TreMes/1-115 ---ATCATTTCAAGAAGCTATACCTTTAATGTTATTAGCAGCGGTTTTAGGTTTACCTGCTATTTTGATCTTGATTACAACTAGAAAAGTCATTTACGTAGCTTGGATGATGGCTTAT
#=GS CryFla/1-104 DE CAUG01000683.1:41023-41126 Cryptococcus flavescens NRRL Y-50378 WGS project CAUG00000000 data, contig NODE_2442_length_249147_cov_46_019089, whole genome shotgun sequence
CryFla/1-104 -------------GAGGCCATCCCGCTCATGCTCCTCGCGGCGGTGCTGGGTCTGCCGGCGGTCTTGATCCTGATCACAACGCGCAAGGTCATTTACGTGGCGTGGATGGTGGTGTA-
#=GS PapTer/1-104 DE JAHXHD010000316.1:11236-11339 Papiliotrema terrestris strain LS28 scaffold-315, whole genome shotgun sequence
PapTer/1-104 --------------AGGCCATCCCGCTCATGCTTCTCGGGGCGGTGCTTGGCCTTCCGGCGGTCTTGATCCTCATCACGACGCGCAAGGTGATCTACGTGGCGTGGATGGTGGTGTAT
//
Image source snRcnk02420-2D target CNL04660, CNAG_05013:
# STOCKHOLM 1.0
#=GF RW van Nues, July 2023
#=GF https://coalispr.codeberg.page/paper
snRcnk02420-2-D/1-21 ------------------------------------agtc-ttgCCGAGATTGGTCAA----------------------------------------------------------
Target-found/1-17 -----------------------------------------AATGGCTTTGATTGGTT----------------------------------------------------------
#=GS CNL06030/1-116 DE protein-nucleus import-related protein AE017352.1:c666359-666244 Cryptococcus neoformans var. neoformans JEC21 chromosome 12 sequence
CNL06030/1-116 TCTTGAACGCGGATGATGCCAAGGCTGCTATTGAGATGTTCAATGGCTTTGATTGGTTTGGTAATGTCCTTGAAGTCCGAGAGGTCAGTGTTCCATCTCCAGCTGGGCTGTCACTT
#=GS CNAG_05013/1-116 DE RNP domain-containing protein CP003823.1:228032-228147 Cryptococcus neoformans var. grubii H99 chromosome 4, complete sequence
CNAG_05013/1-116 TCTTGAACGCGGATGATGCCAAGGCTGCTATTGAGATGTTCAATGGTTTTGATTGGTTTGGTAATGTTCTTGAAGTCCGGGAGGTTAGTCTTCCATCGCCAGCTGGGCTGTCACTT
#=GS WM276/1-116 DE CP000289.1:213718-213833 Cryptococcus gattii WM276 chromosome D, complete sequence
WM276/1-116 TCTTGAATGCGGATGATGCCAAGGCTGCTATCGAGATGTTCAATGGCTTTGATTGGTTTGGTAACGTTCTTGAAGTCCGAGAGGTTAGTCTTTCAACTCCAACTGAGCTTTCGCTT
#=GS R265/1-115 DE CP025760.1:209691-209805 Cryptococcus gattii VGII R265 chromosome 2, complete sequence
R265/1-115 TCTTGAATGCAGATGATGCCAAGGCTGCCATCGAGATGTTCAACGGCTTTGATTGGTTTGGTAACGTTCTTGAAGTCCGAGAGGTTAGTCTTCCAACTCCAACTGAGCTGTCACT-
#=GS TsuWin/1-79 DE XM_019174602.1:941-1019 Tsuchiyaea wingfieldii CBS 7118 RNP domain-containing protein (L198_02453), partial mRNA
TsuWin/1-79 ---TGAACGCGGATGACGCCAAGGCGGCCATCGAAATGTTTAACGGCTTTGACTGGTTCGGCAACGTTCTCGAAGTACGCGA----------------------------------
#=GS CryFlo/1-53 DE RRZH01000002.1:c231187-231135 Cryptococcus floricola strain DSM 27421 chromosome 2, whole genome shotgun sequence
CryFlo/1-53 -----------------------------ATAGAAATGTTCAACGGCTTTGACTGGTTCGGCAACGTTCTCGAAGTGCGCGA----------------------------------
#=GS CryWin/1-53 DE AWGH01000005.1:714951-715003 Cryptococcus wingfieldii CBS 7118 supercont1.5, whole genome shotgun sequence
CryWin/1-53 -----------------------------ATAGAAATGTTTAACGGCTTTGACTGGTTCGGCAACGTTCTCGAAGTACGCGA----------------------------------
#=GS CryAmy/1-53 DE MEKH01000001.1:c206837-206785 Cryptococcus amylolentus CBS 6273 supercont2.1, whole genome shotgun sequence
CryAmy/1-53 -----------------------------ATAGAAATGTTCAACGGCTTTGACTGGTTCGGCAACGTTCTCGAAGTACGCGA----------------------------------
#=GS CryGC4/1-68 DE JALPCA010000038.1:c19348-19281 Cryptococcus sp. GC_Crypt_4 iso00_46_65, whole genome shotgun sequence
CryGC4/1-68 -----------------GCCAAAGCCGCTATCGAGATGTTCAACGGATTCGATTGGTTCGGAAATGTCCTTGAGGTCCGAGAGGT-------------------------------
#=GS TreTag/1-53 DE CAJHEQ010000317.1:c5941-5889 MAG: Tremellales sp. Tagirdzhanova-0007 genome assembly, contig: TREM_317, whole genome shotgun sequence
TreTag/1-53 --------------------------------GAGATGTTCAACGGCTTTGACTGGTTTGGTAATGTTCTCGAAGTCCGAGAGGT-------------------------------
#=GS CrySki/1-59 DE BCHT01000001.1:1184623-1184681 Cryptococcus skinneri DNA, scaffold: scaffold_0, strain: JCM 9039, whole genome shotgun sequence
CrySki/1-59 --------------------------------GAGATGTTCAATGGCTATGACTGGTTTGGCAGTATCCTTGAAGTCCGCGAGGTGAGTGT-------------------------
#=GS KwoMan/1-69 DE ASQF01000037.1:394196-394264 Kwoniella mangroviensis CBS 8886 cont1.37, whole genome shotgun sequence
KwoMan/1-69 -----AACCCCGAGGATGCTCGAGCTGCTATTGAGATGTTCAACGGATTTGATTGGTTCGGCAATATCCTTGAA------------------------------------------
#=GS TreFuc/1-53 DE BRDD01000080.1:152756-152808 Tremella fuciformis NBRC 9317 DNA, KCNB80TF.80, whole genome shotgun sequence
TreFuc/1-53 --------------------------------GAAATGTTCAACGGCTTTGACTGGTTTGGGAATGTCCTTGAAGTTCGCGAGGT-------------------------------
#=GS KocImp/1-57 DE NBSH01000005.1:273964-274020 Kockovaella imperatae strain NRRL Y-17943 BD324scaffold_5, whole genome shotgun sequence
KocImp/1-57 --------------------------------GAAATGTTCCACGGCTTTGACTGGTTTGGCAACATTCTGGAAGTTCGAGAGGTGAGT---------------------------
#=GS KwoHev/1-57 DE ASQB01000034.1:258430-258486 Kwoniella heveanensis BCC8398 cont1.34, whole genome shotgun sequence
KwoHev/1-57 --------------------------------GAGATGTTTAACGGCTTTGACTGGTTCGGCAACATCTTGGAGGTCCGAGAGGTGAGT---------------------------
#=GS KwoSha/1-56 DE NQVO01000050.1:c73775-73720 Kwoniella shandongensis strain CBS 12478 scaffold00050, whole genome shotgun sequence
KwoSha/1-56 --------------------------------GAGATGTTCAACGGCTTCGACTGGTTCGGCAGCATTCTTGAGGTCCGAGAGGTGAG----------------------------
#=GS CryFla/1-56 DE CAUG01000159.1:c61822-61767 Cryptococcus flavescens NRRL Y-50378 WGS project CAUG00000000 data, contig NODE_220_length_79477_cov_46_199150, whole genome shotgun sequence
CryFla/1-56 --------------------------------GAAATGTTCAACGGCTTTGACTGGTTCGGCAACATTCTCGAAGTCCGAGAGGTGAG----------------------------
#=GS BulAlb/1-56 DE CAMYTR010000036.1:63326-63381 Bullera alba genome assembly, contig: jcf7180000012129, whole genome shotgun sequence
BulAlb/1-56 --------------------------------GAGATGTTCCACGGCTTCGATTGGTTTGGCAACATTCTCGAGGTCCGCGAGGTGAG----------------------------
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